The Calibration curve reference article from the English Wikipedia on 24-Apr-2004 (provided by Fixed Reference: snapshots of Wikipedia from wikipedia.org)

Calibration curve

A calibration curve is a graphical display of the functional relationship between the expected value of the observed signal to the analyte amount.

In analytical chemistry, a calibration curve is a standard method for analysis of concentration. The experimenter will create a series of standards across the range of concentrations that are of interest. They must take care that these concentrations are in the detection range of the technique (instrumentation) they are using. These standards will have a precisely known concentration of the element or compound under study. Running each of these standards several times using the chosen technique will produce a series of readings, each set indicative of one of the known concentrations. By plotting these points (reading vs concentration) on a graph, it is possible to plot a line of reading vs concentration across the detection range of that technique. Thus, when the sample is run and a reading obtained, the experimenter can simply refer to the graph to read off the concentration.

Applications

• Analysis of concentration
• Verifying the proper functioning of a sensor device such as an ion-specific electrode
• Determining the basic effects of a control treatment (such as a dose-survival curve in clonogenic assay).

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